In order to study the calculation method of the horizontal seismic force, the two-tiered re-inforced soil retaining walls structure model was constructed and large-scale shaking table tests was per-formed. The geometric similarity ratios of model was deduced by Buckingham π theorem. The 1.8m nu-merical model and the 18m numerical model were established by FLAC3D software. The acceleration am-plification of the two-tiered reinforced soil retaining walls was tested. The results show that, acceleration amplification distribution along height of walls are non-linear. acceleration amplification of the top wall is larger than that of the low retaining wall. the results of shaking table test are smaller than that of the numerical model when model is under the same acceleration. Under different peak acceleration, the peak acceleration is larger, but acceleration amplification effect is more weakened. According to action of the single-tiered reinforced soil retaining wall and the two-tiered reinforced soil retaining wall under different accelerations, the acceleration amplification coefficient of two-tiered reinforced soil retaining wall suf-fered from horizontal seismic loading is defined. the formula for calculating horizontal seismic force of two-tiered reinforced soil retaining wall is proposed. The formula can provide theoretical support for seismic design and post-earthquake calculation of reinforced soil retaining wall.